![]() ![]() Neonatal sepsis is the most common cause of neonatal death 2 and, therefore, early diagnosis 3 and treatment is very important. Neonatal septicaemia is a leading cause of morbidity but also in mortality of infants not only in the developing but also in developed countries and is responsible for 30-50 per cent deaths in developing countries 1. The results of our study demonstrate that PCR-RFLP having a rapid turnaround time may be useful for the early diagnosis of culture negative neonatal sepsis. Eighty of the 97 neonates had prior exposure to antibiotics. The sensitivity of PCR was found to be 82.3 per cent with a negative predictive value of 85.7 per cent. ![]() was isolated from one case but detected in eight cases by PCR-RFLP. ![]() was isolated from four samples by culture but was detected by PCR-RFLP in five cases while Acinetobacter spp. Staphylococcus aureus was the most common organism detected with both methods. Results:īacterial DNA was detected by 16S rRNA gene PCR in 55 cases, while BacT/Alert culture was positive in 34 cases. Methods:īlood samples from 97 neonates were evaluated for diagnosis of neonatal sepsis using BacT/Alert (automated blood culture) and PCR-RFLP. In this study, we used restriction fragment length polymorphism (RFLP) of amplified 16S rRNA gene to identify the organisms involved in neonatal sepsis and compared the findings with blood culture. Though polymerase chain reaction (PCR) based detection of bacterial 16S rRNA gene has been reported earlier, this does not provide identification of the causative agent. The difficulties in diagnosis of neonatal sepsis are due to varied clinical presentation, low sensitivity of blood culture which is considered the gold standard and empirical antibiotic usage affecting the outcome of results. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |